Histopathological, Hemochromatotic Hypercholesterolemic, and Androgenic Effects of Escravos Crude oil on the Testis in Male Chinchilla Rabbits.
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Date
2014-06
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Abstract
Aim: This study aimed to investigate the effects of Escravos crude oil on serum
concentrations of testosterone, total cholesterol, body weight, relative weight and microanatomical
architecture of the testis using male Chinchilla Rabbits.
Place and Duration of Study: This study was carried out at the Department of Medical
Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus between May and June
2013 (28 days).
Methodology: A total of thirty male Chinchilla Rabbits aged 12 to 14 weeks and weighing
1.2kg to 1.45kg was used. Crude oil was administered orally at the doses of 15, 20, 25
and 30mg/kg body weight to groups designated B, C, D and E respectively for 28 days,
while group A was given normal saline. Serum concentration of testosterone and total cholesterol were estimated using the microplate enzyme immunoassay and enzymatic
end point methods, respectively. The SPSS software (version 20) was used for the
statistical analysis and the result expressed in mean ± SD.
Result: The results showed dose dependent effects on the hormone and biochemical
assays, especially at the concentration of 25mg/kg body weight of the administered crude
oil. Significant increases in serum concentrations of testosterone (0.32+/-0.05 to 0.46+/-
0.14) and total cholesterol (1.35+/-0.17 to 1.76+/-0.15) concentrations (p≤.05), and
insignificant increase (p≥.05) in the relative weight of the testis (4.80+/-0.40 to 6.50+/-
0.90) were observed. The histology of the testes revealed hypertrophy of the seminiferous
tubules, atrophy of the basal lamina and interstitial cells, and hemochromatosis. The
histological findings agree with the hormonal and biochemical findings.
Conclusion: These findings suggest that Escravos crude oil might be a potential
endocrine disruptor and toxic substance which can affect the micro-anatomical
architecture of the testis.
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Keywords
Escravos crude oil, total cholesterol, testosterone, hemochromatosis, histopathology, testis, male chinchilla Rabbit
Citation
Okoye J O, Ude T, Ibekailo S N, Awalu C J. Histopathological, Hemochromatotic Hypercholesterolemic, and Androgenic Effects of Escravos Crude oil on the Testis in Male Chinchilla Rabbits. British Biotechnology Journal. 2014 Jun; 4(6): 649-658.