Respiratory Burst Enzymes and Oxidant-antioxidant Status in Nigerian Children with Sickle Cell Disease.
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Date
2014-03
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Abstract
Aim: To measure respiratory burst enzymes, pro-oxidants, antioxidants and red cell
indices in Nigerian children with sickle cell disease (HbSS) below five years of age and
compared with apparently healthy children with normal haemoglobin (HbAA).
Method: A total of 45 subjects were recruited which included 23 children (age range 10 –
48 months) with HbSS and 22 children (age- and sex- matched) with HbAA. Blood
samples were collected and red cell indices were determined using automated
haematology analyser while serum superoxide dismutase (SOD), glutathione peroxidise
(GSH-Px) and myeloperoxidase (MPO) activities were measured using ELISA kits. Serum
malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione S transferase (GST),
catalase (Cat), xanthine oxidase (XO) and glutathione (GSH) were measured with
colorimetric techniques. MPO, SOD and Cat represented respiratory burst enzymes;
MDA, H2O2 and XO were measured as pro-oxidants while GSH, GST and GSH-Px were the measured antioxidants.
Results: Mean concentration of malondialdehyde was significantly reduced
(5.56±1.12nmol/L vs. 6.46±1.11nmol/L, P=.04) in HbSS children compared with HbAA
children. Similarly, mean serum activity of myeloperoxidase in HbSS children was
significantly reduced compared with HbAA children (66.12±13.34U/mL vs
77.02±13.54U/mL, P=.03). However, there were no significant differences in mean
concentration of serum glutathione, hydrogen peroxide; serum activities of glutathione
peroxidase, catalase, superoxide dismutase, xanthine oxidase and glutathione S
transferase in HbSS children compared with HbAA children
Conclusion: HbSS children in this population did not demonstrate raised oxidative stress.
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Keywords
Antioxidants, free radicals, respiratory burst, sickle cell disease, children
Citation
Adelakun Ayodele, Ajani Olumide, Ogunleye Temitope, Disu Elizabeth, Kosoko Ayokulehin, Arinola Ganiyu. Respiratory Burst Enzymes and Oxidant-antioxidant Status in Nigerian Children with Sickle Cell Disease. British Biotechnology Journal. 2014 Mar; 4(3): 270-278.