In vitro Propagation of Caralluma diffusa (Wight) N.E.Br.
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Date
2014-02
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Abstract
An efficient protocol is described for the rapid in vitro multiplication of an endangered
medicinal plant, Caralluma diffusa, via enhanced axillary bud proliferation from nodal
explants collected from young shoots of six-months-old plant. The physiological effects of
growth regulators Benzylamino purine (BAP), Thidiazuron (TDZ), Kinetin (KIN), α-
Naphthalene acetic acid (NAA) full strength of Murashige and Skoog’s (MS) medium on in
vitro propagation were investigated. The highest number of shoots in initiation (4.16±0.30)
and in subculture (17.33±0.33) and the maximum average shoot length (4.17±0.03cm)
were recorded on MS medium supplemented with BAP (4.44 μM) and TDZ (0.90 μM) at
pH 5.8. Rooting was best achieved on MS medium augmented with NAA (5.37 μM). The
plantlets regenerated in vitro with well-developed shoot and roots were successfully
established in pots containing decomposed coir waste, vermiculite and garden soil
(1:1:1ratio) and grown in a shade house with 93.83% survival rate.
Description
Keywords
In vitro, micropropagation, Caralluma diffusa, endangered
Citation
Kalimuthu K, Kalaiyarasi K, Prabakaran R, Sasikala T. In vitro Propagation of Caralluma diffusa (Wight) N.E.Br. British Biotechnology Journal. 2014 Feb; 4(2): 164-172.