Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix.
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Date
2014-02
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Abstract
Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white
matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed
batch study.
Methodology: Effect of pH, matrix size, enzyme concentration, contact time and
temperature on the binding of cellulase on egg white matrix was studied. Desorption
studies was carried with variable pH, sodium chloride and ammonium sulphate system to
desorbs the cellulase for the egg white matrix.
Results: Equilibrium isotherms for the adsorption of the cellulase enzyme on egg white
matrix were well fitted to Freundlich and Langmuir isotherm models. The adsorption
process has been found endothermic in nature and thermodynamic parameters, Gibb’s
free energy (ΔGº), change in enthalpy (ΔHº) and change in entropy (ΔSº) have been
calculated as -13276.21, 2025.47 and 698.64 (KJ mol-1) respectively. Thermodynamic
studies revealed that the adsorption process is spontaneous and endothermic in nature.
In desorption studies it was concluded that maximum elution (66.8% was obtained for
cellulase enzyme with 0.3M (NH4)2SO4 with respect to different pH and sodium chloride
solution. Cellulase enzyme obtained after desorption process has maximum purification
fold of 4.25 with 60% recovery.
Conclusion: The results from the current research suggest that egg white can be used for partial purification of cellulase enzyme with less cost.
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Keywords
Adsorption, cellulose, isotherm, egg white matrix
Citation
Singh Jagdish, Kaur Manpreet. Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix. British Biotechnology Journal. 2014 Feb; 4(2): 108-115.