Preparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two- Dimensional Gel Electrophoresis.
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Date
2013-04
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Abstract
Aims: Find a suitable method for the protein extraction from flower buds of Solanum
lycopersicum.
Study Design: Compare some kinds of protein extraction methods and find the best one
among them suitable to tomato flower buds.
Place and Duration of Study: Biological Science and Technology College, between
June 2010 and July 2011.
Methodology: The proteins for electrophoresis were extracted using different methods,
such as trichloroacetic acid /acetone (TCA/acetone), Sodium dodecyl sulfate (SDS), Trissaturated
phenol (Tris-Phen), Phenol/SDS and Direct lysis method. After silver staining,
different patterns of protein spots were observed in the gels.
Results: Few spots were found by SDS and Phenol/SDS extractions, more spots by immediate dissolution but the most impurities, less protein productivity though more spots
by Tris-Phen extractions, and more protein productivity and better apart effect by
TCA/acetone. The 2-DE image background was the clear and the protein spots were the
most by TCA/acetone method.
Conclusion: TCA/acetone method is much more suitable as extraction method for protein
two-dimensional electrophoresis of tomato flower buds.
Description
Keywords
Flower buds, protein extraction, Solanum lycopersicum, two-dimensional gel electrophoresis
Citation
Zhao Xiaocui, Ren Jingqi, Cui Na, Fan Haiyan, Yu Guangchao, Li Tianlai. Preparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two- Dimensional Gel Electrophoresis. British Biotechnology Journal. 2013 Apr; 3(2): 183-190.