Isolation, characterization and identification of bacteria from coolant oils.
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Date
2011-07
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Abstract
Coolants are hydrocarbons, used to lubricate parts of machines for smooth performance.
While in use, a coolant quickly gets contaminated with foreign materials, making it less effective and
unpleasant odors are developed due to microbial action. Hence coolants need to be replaced frequently.
The expense of disposing used coolants and replacing it with fresh coolants adds significantly to the
manufacturing cost. The present study is focused on isolation, identification and characterization of
coolant oil contaminating bacteria as an initial step to solve these contamination problems. Used and
unused samples of coolant were collected from oil stations, auto mechanic workshops and steel industry
for the isolation of the contaminants. Ten dominant bacterial isolates of the genus Pseudomonas,
Staphylococcus, Micrococcus, Salmonella, Cellobiococcus and Pneumonia were identified by
morphology, biochemical tests and PIB tool. Isolates were subjected to four different media, various pH
and temperatures for characterization of optimal conditions of growth. Pseudomonas pseudomallei,
Micrococcus luteus 3, Micrococcus varians and Salmonella ferlac were observed in mineral, synthetic
and aerobic media, Staphylococcus hyicus, Cellobiococcus species and Staphylococcus intermedius in
synthetic and aerobic fermentation media and Pseudomonas cepacia, Pseudomonas piketti in mineral salt
and aerobic fermentation media. The ten isolates showed optimal growth at different temperatures
between 20°C and 90°C and different pH, ranging from acidic to alkaline. In conclusion, the used coolants
harbor hazardous pathogens such as Pseudomonas species which multiply rapidly and survive high
temperatures. These isolates could be targeted for further studies on development of antidotes as a
solution to the coolant contamination problems.
Description
Keywords
Coolant oil, Biochemical tests, Oil degrading bacteria, PIB tool, Bacterial identification
Citation
Badrunnisa S, Shantaram Manjula, Pai Vinitha Ramanath. Isolation, characterization and identification of bacteria from coolant oils. International Journal of Applied Biology and Pharmaceutical Technology. 2011 Jul-Sept; 2(3): 444-452.