Detection of Metabolites of Vecuronium Bromide in Visceral Samples Solves A Typical Death Mystery- (Toxicokinetics- Studies Using Gc-Ms Technique).
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Date
2015-01
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Abstract
Introduction: On Sudden death of a medical student (20 year old girl) of National Institute of Medical Science autopsy materials
along with site of injection were sent to F.S.L. It was informed during investigation that the girl took tetanus vaccine before
death. In crime scene investigation Forensic Team observed that it was a case of gross negligence of dispensing wrong injection.
Material: Tests were performed on viscera material viz., liver, spleen, kidney, lungs, brain, skin and blood etc. Mystery of suspected
death was solved when a new GC-MS application was designed to get the unknown drug and various fragments of extracted
material were studied. Method: A new method was developed on gas-chromatography-mass spectrophotometer and TL.C using
various solvent systems is explained. Results: Metabolite fragments of vecuronium bromide a muscle relaxant were surprisingly
observed in Viscera material, Blood sample and Skin piece from Leftt cubital fossa from this young girl, whose death is questioned.
Conclusion: Structural elucidations of fragments provide a new approach to toxicokinetics. Th e explanation of fragments obtained
were structurally compared with other neuromuscular blocking groups like atracurium and succinyl choline Th e presence of
bromide attached cholest-5-en-Br, hydroxylated cholest-5-en-ol as hydroxylated product, acetylated fragments as cholest-5-enacetate
and piperidone-2-one present in visceral samples indicates structural part obtained from vecuronium bromide drug. Th ese
metabolites studies makes the case studies highly informative. Beside this new method of extractions, TLC systems and colouring
reagents are also explained.
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Chromatography,, Mass spectrometry, Thin layer chromatography, Toxico-kinetics
Citation
Khanna Rakhi, Sharma R S. Detection of Metabolites of Vecuronium Bromide in Visceral Samples Solves A Typical Death Mystery- (Toxicokinetics- Studies Using Gc-Ms Technique). Acta Medica International. 2015 Jan-June; 2(1): 125-133.