Modulation of phagolysosome maturation by bacterial tlyA gene product.
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Date
2014-12
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Abstract
The pathogenic traits of TlyA proteins of Mycobacterium tuberculosis are not known. Expressions of TlyA in bacteria
that do not express endogenous TlyA adhere better to RAW264.7 macrophages and get phagocytosed efficiently. The
internalized bacteria avoid acidification to the extent of >65% in the case of both TlyA-expressing E. coli and
M. smegmatis. Consistent with this observation, we have observed decreased co-localizaton of Lysosomal Membrane
Associated Protein-1 (~35%), Early Endosomal Antigen-1 (~34%), Rab5 (~30%) and Rab7 (~35%) and enhanced colocalizaton
of Rab14 (~80%) on both TlyA-expressing bacteria as well as on TlyA-coated latex beads. These results
suggest that the mycobacterial TlyA, in general, can modulate phagolysosome maturation pathway immediately after
entry into macrophages, while other important molecules may aid the bacterium for long-term, intracellular survival at
later point of time.
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Keywords
Intracellular survival, phagolysosome maturation, phagosome acidification, Rab-GTPase modulation, tlyA gene product
Citation
Mittal Ekansh, Kumar Santosh, Rahman Aejazur, Krishnasastry Musti V. Modulation of phagolysosome maturation by bacterial tlyA gene product. Journal of Biosciences. 2014 Dec; 39 (5): 821-834.