Hypoxia influences expression profile of Pleckstrin homology-like domain, family A, member 2 in Indian catfish, Clarias batrachus (Linnaeus, 1758): A new candidate gene for hypoxia tolerance in fish.
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Date
2014-06
Authors
Mohindra, Vindhya
Tripathi, Ratnesh K
Yadav, Prabhaker
Singh, Rajeev K
Lal, Kuldeep K
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Abstract
Several physiologically important genes were found to be regulated by hypoxia at the transcriptional level. The Pleckstrin
homology-like domain, family A, member 2 (PHLDA2) gene was previously identified as an imprinted gene. The present
study was aimed to determine the structure of complete cDNA and the deduced protein of PHLDA2 along with analysing
the changes in its mRNA expression in Clarias batrachus tissues under hypoxic conditions. The complete cDNA of
CbPHLDA2 gene consisted of 1009 nucleotides with an open reading frame of 417 nucleotides. The deduced CbPHLDA2
protein of 139 amino acids shared high homology with PHLD2A of other fishes as well as that of vertebrates. Importantly,
a single amino acid (asparagine/lysine) insertion was identified in the PH domain of CbPHLDA2 and other fishes, which
was absent in other vertebrates studied. Furthermore, under normoxic conditions, CbPHLDA2 was constitutively
expressed with varying levels in analysed tissues. Short- and long-term hypoxia exposure resulted in significant changes
in the expression of CbPHLDA2 in liver, spleen, head kidney, brain and muscle in a time-dependent manner. The results
suggested that CbPHLDA2 might play an important role for adaptive significance under hypoxia.
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Keywords
CbPHLDA2, Clarias batrachus, hypoxia, differential expression
Citation
Mohindra Vindhya, Tripathi Ratnesh K, Yadav Prabhaker, Singh Rajeev K, Lal Kuldeep K. Hypoxia influences expression profile of Pleckstrin homology-like domain, family A, member 2 in Indian catfish, Clarias batrachus (Linnaeus, 1758): A new candidate gene for hypoxia tolerance in fish. Journal of Biosciences. 2014 Jun; 39 (3): 433-442.