Hpa1 harpin needs nitroxyl terminus to promote vegetative growth and leaf photosynthesis in Arabidopsis.
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Date
2014-03
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Abstract
Hpa1 is a harpin protein produced by Xanthomonas oryzae, an important bacterial pathogen of rice, and has the
growth-promoting activity in plants. To understand the molecular basis for the function of Hpa1, we generated an
inactive variant protein, Hpa1ΔNT, by deleting the nitroxyl-terminal region of the Hpa1 sequence and compared
Hpa1ΔNT with the full-length protein in terms of the effects on vegetative growth and related physiological responses
in Arabidopsis. When Hpa1 was applied to plants, it acted to enhance the vegetative growth but did not affect the
floral development. Enhanced plant growth was accompanied by induced expression of growth-promoting genes in
plant leaves. The growth-promoting activity of Hpa1 was further correlated with a physiological consequence shown
as promoted leaf photosynthesis as a result of facilitated CO2 conduction through leaf stomata and mesophyll cells. On
the contrary, plant growth, growth-promoting gene expression, and the physiological consequence changed little in
response to the Hpa1ΔNT treatment. These analyses suggest that Hpa1 requires the nitroxyl-terminus to facilitate CO2
transport inside leaf cells and promote leaf photosynthesis and vegetative growth of the plant.
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Keywords
Arabidopsis, CO2 transport, Hpa1, photosynthesis, plant growth enhancement, Xanthomonas oryzae
Citation
Li Xiaojie, Han Liping, Zhao Yanying, You Zhenzhen, Dong Hansong, Zhang Chunling. Hpa1 harpin needs nitroxyl terminus to promote vegetative growth and leaf photosynthesis in Arabidopsis. Journal of Biosciences. 2014 Mar; 39(1): 127-137.