Analysis of Rheb in the cellular slime mold Dictyostelium discoideum: Cellular localization, spatial expression and overexpression.
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Date
2014-03
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Abstract
Dictyostelium discoideum encodes a single Rheb protein showing sequence similarity to human homologues of Rheb.
The DdRheb protein shares 52% identity and 100% similarity with the human Rheb1 protein. Fluorescence of Rheb
yellow fluorescent protein fusion was detected in the D. discoideum cytoplasm. Reverse transcription-polymerase
chain reaction and whole-mount in situ hybridization analyses showed that rheb is expressed at all stages of
development and in prestalk cells in the multicellular structures developed. When the expression of rheb as a fusion
with lacZ was driven under its own promoter, the β-galactosidase activity was seen in the prestalk cells. D. discoideum
overexpressing Rheb shows an increase in the size of the cell. Treatment of the overexpressing Rheb cells with
rapamycin confirms its involvement in the TOR signalling pathway.
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Keywords
Dictyostelium, Rheb, TOR signalling
Citation
Swer Pynskhem Bok, Bhadoriya Pooja, Saran Shweta. Analysis of Rheb in the cellular slime mold Dictyostelium discoideum: Cellular localization, spatial expression and overexpression. Journal of Biosciences. 2014 Mar; 39(1): 75-84.