Amplicon restriction patterns associated with nitrogenase activity of root nodules for selection of superior Myrica seedlings.
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Date
2013-11
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Abstract
Trees of Myrica sp. grow abundantly in the forests of Meghalaya, India. These trees are actinorhizal and harbour
nitrogen-fixing Frankia in their root nodules and contribute positively towards the enhancement of nitrogen status of
forest areas. They can be used in rejuvenation of mine spoils and nitrogen-depleted fallow lands generated due to slash
and burn agriculture practiced in the area. We have studied the association of amplicon restriction patterns (ARPs) of
Myrica ribosomal RNA gene and internal transcribed spacer (ITS) region and nitrogenase activity of its root nodules.
We found that ARPs thus obtained could be used as markers for early screening of seedlings that could support strains
of Frankia that fix atmospheric nitrogen more efficiently.
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Keywords
Amplicon restriction pattern, molecular markers, Myrica, nitrogenase activity
Citation
Yanthan Mhathung, Misran Arvind K. Amplicon restriction patterns associated with nitrogenase activity of root nodules for selection of superior Myrica seedlings. Journal of Biosciences. 2013 Nov; 38(4): 789-795.