Bioluminescent bioreporter for assessment of arsenic contamination in water samples of India.
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Date
2013-06
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Abstract
In the present study the most efficient R-factor controlling the ars operon was selected after screening of 39
Escherichia coli isolates by minimum inhibitory concentration test (MIC) studies from water samples of different
geographical locations of India. Among all, strain isolated from Hooghly River (West Bengal) was found to have
maximum tolerance towards arsenic and was further used for the development of bioreporter bacteria. Cloning of the
ars regulatory element along with operator-promotor and luxCDABE from Photobacteria into expression vector has
been accomplished by following recombinant DNA protocols. The bioreporter sensor system developed in this study
can measure the estimated range of 0.74–60 μg of As/L and is both specific and selective for sensing bioavailable As.
The constructed bacterial biosensor was further used for the determination of arsenic ion concentration in different
environmental samples of India.
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Keywords
ars operon, arsenic, bacterial sensor, bioluminescence, bioreporter
Citation
Sharma Pratima, Asad Shahzada, Ali Arif. Bioluminescent bioreporter for assessment of arsenic contamination in water samples of India. Journal of Biosciences. 2013 June; 38(2): 251-258.