Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton.
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Date
2013-03
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Abstract
Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory
to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA
that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of
adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a
cotton breeding population and in hybrid seed purity testing.
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Keywords
Cotton, cry1Ac, genomic DNA isolation, high-resolution melting curve analysis, radicle tip, seed purity testing
Citation
Rao P Srinivasa, Kumar P Sateesh, Sonti Ramesh V. Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton. Journal of Biosciences. 2013 Mar; 38(1): 167-172.