Rhamnolipid but not motility is associated with the initiation of biofilm seeding dispersal of Pseudomonas aeruginosa strain PA17.
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Date
2013-03
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Abstract
Seeding dispersal is an active detachment exhibit in aging Pseudomonas aeruginosa biofilm. Yet, effect factors of this
process in the biofilm of clinical isolated mucoid P. aeruginosa strain remain to be better characterized. In our
previous work, one mucoid P. earuginosa strain PA17 was isolated from a patient with recurrent pulmonary infection.
In this study, confocal scanning laser microscope combined with LIVE/DEAD viability staining revealed that PA17
biofilm exhibited earlier seeding dispersal than non-mucoid PAO1. We further compared the motility and the
expression of motility-associated gene during biofilm development between PA17 and PAO1. PA17 was found to
be impaired in all three kinds of motility compared to PAO1. Moreover, we investigated the expression of
rhamnolipid-associated genes in PA17 and PAO1 biofilm. The expression of these genes was in accordance with
the process of seeding dispersal. Our results indicated that rhamnolipid but not motility is associated with the initiation
of seeding dispersal of PA17 biofilm.
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Keywords
Biofilm, dispersal, motility, Pseudomonas aeruginosa, rhamnolipid
Citation
Wang Jingjing, Yu Bing, Tian Deying Ni Ming. Rhamnolipid but not motility is associated with the initiation of biofilm seeding dispersal of Pseudomonas aeruginosa strain PA17. Journal of Biosciences. 2013 Mar; 38(1): 149-156.