Synergistic activity of ofloxacin and ornidazole on biomedical fabrics against nosocomial pathogens.
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Date
2011-04
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Abstract
Nosocomial infections in the hospitals disseminated from the cotton fabrics of health
care professionals and patients leads to severe complications like respiratory, gastrointestinal and
urinary tract infections. Since the hospital based textile materials like nylon and polyester has good
surface properties, it can harbour large number of microorganisms. Hence in this study, two different
antibacterial drugs showing synergistic properties were attached to different fabricsusing tocopherol
acetate as a cross-linker with the aim that, treated fabric could act as barriers against transmission of
challenge organisms. Inorder to decrease the drug resistant property of the nosocomial pathogens, a
fluoroquinolone and a nitroimidazole compounds were mixed at suitable composition based on their
synergistic behaviour. Both the compounds were modified to act as reactive dyes and were covalently
bonded to the surface of nylon and polyester in order to impart antibacterial properties. The assay
used for measuring antibacterial properties was based on the AATCC Test Method-100. The treated
fabric was also subjected to multiple washings to determine its durability based on the AATCC Test
Method-124. To determine the mode of action of these drugs, DNA of the drug exposed and
unexposed challenge organisms were extracted and analysed by agarose gel electrophoresis. The
difference in the number of viable bacteria after ‘0’ contact time and 18 hours contact time with
treated fabrics were statistically calculated with P<0.05 considered significant.
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Keywords
Reactive dye method, AATCC-100, AATCC-124, beta-cyclodextrin, agarose gel electrophoresis
Citation
Rajendran R, Elayarajah B, Venkatrajah B, Balakumar C, Asa Sudhakar, Janiga.P K, Devika S. Synergistic activity of ofloxacin and ornidazole on biomedical fabrics against nosocomial pathogens. International Journal of Applied Biology and Pharmaceutical Technology. 2011 Apr-June; 2(2): 434-447.