Aggregation properties of a short peptide that mediates amyloid fibril formation in model proteins unrelated to disease.
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Date
2011-09
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Abstract
Short peptides have been identified from amyloidogenic proteins that form amyloid fibrils in isolation. The
hexapeptide stretch 21DIDLHL26 has been shown to be important in the self-assembly of the Src homology 3 (SH3)
domain of p85α subunit of bovine phosphatidylinositol-3-kinase (PI3-SH3). The SH3 domain of chicken brain α-
spectrin, which is otherwise non-amyloidogenic, is rendered amyloidogenic if 22EVTMKK27 is replaced by
DIDLHL. In this article, we describe the aggregation behaviour of DIDLHL-COOH and DIDLHL-CONH2. Our
results indicate that DIDLHL-COOH and DIDLHL-CONH2 aggregate to form spherical structures at pH 5 and 6. At
pH 5, in the presence of mica, DIDLHL-CONH2 forms short fibrous structures. The presence of NaCl along with
mica results in fibrillar structures. At pH 6, DIDLHL-CONH2 forms largely spherical aggregates. Both the peptides
are unstructured in solution but adopt β-conformation on drying. The aggregates formed by DIDLHL-COOH and
DIDLHL-CONH2 are formed during drying process and their structures are modulated by the presence of mica and
salt. Our study suggests that a peptide need not have intrinsic amyloidogenic propensity to facilitate the selfassembly
of the full-length protein. The propensity of peptides to form self-assembled structures that are nonamyloidogenic
could be important in potentiating the self-assembly of full-length proteins into amyloid fibrils.
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Keywords
Amyloid fibrils, atomic force microscopy, non-fibrillar structures, peptide self-association, SH3 domain
Citation
Chaudhary Nitin, Singh Shashi, Nagaraj Ramakrishnan. Aggregation properties of a short peptide that mediates amyloid fibril formation in model proteins unrelated to disease. Journal of Biosciences. 2011 Sep; 36 (4): 679-689.