Proteomic identification of CIB1 as a potential diagnostic factor in hepatocellular carcinoma.
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Date
2011-09
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Abstract
Hepatocellular carcinoma (HCC), among the most common malignancies worldwide, remains a major threat to public
health, and there is an urgent need to identify novel biomarkers for diagnosis, prognosis and targets for anti-cancer
treatment. In this study, two-dimensional polyacrylamide gel electrophoresis coupled with ESI-Q-TOF MS/MS analysis
was used to identify differentially expressed proteins among the HCC tumour centre, tumour margin and nontumourous
liver tissues. In total, 52 spots with significant alteration were positively identified byMS/MSanalysis. Altered expression
of representative proteins, including CIB1, was validated by Western blotting. Immunostaining suggested an increase
tendency of CIB1 expression from nontumourous liver tissue to tumour centre. Knockdown of CIB1 expression by RNA
interference led to the significant suppression of the cell growth in hepatoma HepG2 cells. These data suggest that CIB1
may be used as a novel prognostic factor and possibly an attractive therapeutic target for HCC.
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Keywords
CIB1, 2-DE, hepatocellular carcinoma, proteomics, proliferation
Citation
Junrong Tong, Huancheng Zhou, Feng He, Yi Gao, Xiaoqin Yang, Zhengmao Luo, Hong Zhang, Jianying Zeng, Yin Wang, Yuanyang Huang, Jianlin Zhang, Longhua Sun, Guolin He. Proteomic identification of CIB1 as a potential diagnostic factor in hepatocellular carcinoma. Journal of Biosciences. 2011 Sep; 36 (4): 659-668.