In vivo NMR study of yeast fermentative metabolism in the presence of ferric irons.
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Date
2011-03
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Abstract
Mathematical modelling analysis of experimental data, obtained with in vivo NMR spectroscopy and 13C-labelled
substrates, allowed us to describe how the fermentative metabolism in Saccharomyces cerevisiae, taken as eukaryotic
cell model, is influenced by stress factors. Experiments on cellular cultures subject to increasing concentrations of ferric
ions were conducted in order to study the effect of oxidative stress on the dynamics of the fermentative process. The
developed mathematical model was able to simulate the cellular activity, the metabolic yield and the main metabolic
fluxes occurring during fermentation and to describe how these are modulated by the presence of ferric ions.
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Keywords
Ethanol yield, in vivo NMR, iron metabolism, mathematical modelling, oxidative stress, Saccharomyces cerevisiae
Citation
Ricci Maso, Martini Silvia, Bonechi Claudia, Braconi Daniela, Santucci Annalisa, Rossi Claudio. In vivo NMR study of yeast fermentative metabolism in the presence of ferric irons. Journal of Biosciences. 2011 Mar; 36(1): 97-103.