Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames.
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2010-12
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Abstract
In Neurospora crassa, crosses between normal sequence strains and strains bearing some translocations can yield
progeny bearing a duplication (Dp) of the translocated chromosome segment. Here, 30 breakpoint junction sequences
of 12 Dp-generating translocations were determined. The breakpoints disrupted 13 genes (including predicted genes),
and created 10 novel open reading frames. Insertion of sequences from LG III into LG I as translocation T(UK8-
18) disrupts the eat-3 gene, which is the ortholog of the Podospora anserine gene ami1. Since ami1-homozygous
Podospora crosses were reported to increase the frequency of repeat-induced point mutation (RIP), we performed
crosses homozygous for a defi ciency in eat-3 to test for a corresponding increase in RIP frequency. However, our
results suggested that, unlike in Podospora, the eat-3 gene might be essential for ascus development in Neurospora.
Duplication–heterozygous crosses are generally barren in Neurospora; however, by using molecular probes developed
in this study, we could identify Dp segregants from two different translocation–heterozygous crosses, and using these
we found that the barren phenotype of at least some duplication–heterozygous crosses was incompletely penetrant.
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Singh Parmit K, Iyer Srividhya V, Sowjanya T Naga, Raj B Kranthi, Kasbekar Durgadas P. Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames. Journal of Biosciences. 2010 Dec; 35(4): 539-546.