Transcription pattern of UL131A-128 mRNA in clinical strains of human cytomegalovirus.
Loading...
Date
2010-09
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Human cytomegalovirus (HCMV) mRNA was obtained from human embryonic lung fi broblast cells infected
by HCMV clinical strains from urine samples of infants at different kinetic periods. The cDNA of UL131A-128
mRNAs was amplifi ed using reverse transcription-polymerase chain reaction (RT-PCR) and analysed by sequencing.
Meanwhile, clones containing UL131A-128 transcripts in an HCMV cDNA library of a clinical strain were selected
and sequenced. It was demonstrated that UL131A-128 mRNA was expressed with immediately early, early and late
kinetics. Sequences obtained by RT-PCR showed that the UL131A gene consisted of two exons and the coding region
of the UL130 gene was not interrupted by any intron in the region as reported earlier. However, the transcript of the
UL128 gene showed two patterns: one pattern consisted of three exons as reported earlier; the other contained the three
exons and also the fi rst intron. Moreover, the above characteristics of UL131A-128 spliced transcripts were confi rmed
by the sequences of clones selected from the HCMV cDNA library. Our results demonstrated that the UL131A, UL130
and UL128 genes were transcribed with the 3′-coterminal, although the initiation points of their mRNA may be
different. The variation in the transcripts found in our study indicated the complex nature of transcription of UL131A-
128 genes in clinical strains of HCMV.
Description
Keywords
Citation
Sun Zhengrong, Ren Gaowei, Ma Yanping, Wang Ning, Ji Yaohua, Qi Ying, Li Mali, He Rong, Qiang Ruan. Transcription pattern of UL131A-128 mRNA in clinical strains of human cytomegalovirus. Journal of Biosciences. 2010 Sep; 35(3): 365-370.