Accumulation of ammonia in culture broth by wild-type nitrogen-fixing bacterium, stenotrophomonas maltophilia.
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Date
2011-01
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Abstract
Stenotrophomonas maltophilia was isolated from paddy field of Kyaukse
District, Mandalay Division, Myanmar. Nitrogen fixing activity of S. maltophilia was
detected in nitrogen free mineral medium supplemented with different carbon sources. S.
maltophilia accumulated highest amounts of ammonia in glucose supplemented medium. But
their nitrogen fixing activity was best at 0.7% glucose concentration. When 0.5609 ppm of
glucose still remained in the medium after 24 hours incubation, no ammonia was detected.
After incubation of 32 hours, ammonia began to accumulate and the amount of 0.5 ppm of
ammonia was detected but no sugar remained in the medium. Highest amount of ammonia (2
ppm) was accumulated in the medium after 48 hour incubation. So we found that ammonium
accumulation began when no sugar remained in the medium, supposing that sugar plays a key
role in ammonia accumulation.
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Keywords
Ammonium accumulation, carbon sources, nitrogen fixing bacteria, Stenotrophomonas maltophilia
Citation
Yu San San, Latt Zaw Ko, Kyaw Ei Phyu, Ly Tin Mar. Accumulation of ammonia in culture broth by wild-type nitrogen-fixing bacterium, stenotrophomonas maltophilia. International Journal of Applied Biology and Pharmaceutical Technology. 2011 Jan-Mar; 2(1): 72-77.