In vitro modulation of adherence and invasion ability of enteroinvasive escherichia coli by different viruses.
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Date
2010-11
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Abstract
Studies on the relationship between viral and bacterial infections showed that in
the context of viral infections the immunity of host organism is reduced temporarily, increasing
the incidence of bacterial infections, like faster bacterial colonization of immunocompromised
bodies, by increasing the level of expression of epithelial cell receptor for bacterial adesins.
Modulation of viruses infected host cells signaling may also induce changes in the
cytoscheleton, which may result in the increase / decrease invasion capacity of bacterial cells.
Enteroinvasive Escherichia coli causes intestinal infections exploiting host cell function, which
include the invasion into non-phagocytic eukaryotic cells such as epithelial and endothelial
cells and associated host cell actin cytoskeletal rearrangements. One of our aims was to
investigate the in vitro adherence and invasion capacity induced by an diarhhoeal
enteroinvasive Escherichia coli strain in the presence of different viral strains: Vaccinia virus
(Poxviridae), measles virus (Paramyxoviridae II); echovirus 32 (Picornaviridae) and Herpes
simplex virus 1 (Herpesviridae). The viral adsorption on HeLa cells was done for six hours at
370C, followed by the evaluation of bacterial adherence and invasion to HeLa cells performed
by the adapted Cravioto’s method and gentamycin protection assay. Viral preinfection of the
cellular substrate induced an increased bacterial adherence index, as well as changes in the
adherence pattern from diffuse tu aggregative. In exchange, the general effect of viral infection
on invasive bacterial capacity was the decrease of invasive ability. In conclusion, viral preinfection
of the susceptible substrate influenced the adherence and invasion ability of
enteroinvasive E. coli bacterial strain, as observed by the intensification of the adherence
capacity, explaining the increased incidence of bacterial infections after viral infections, as well
as faster bacterial colonization of immunocompromised hosts and by reducing the invasive
capacity of epithelial cell by bacterial strains, pleading for increased incidence of extracellular
pathogenic organisms in post-viral infections.
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Keywords
Enteroinvasive Escherichia coli, viral infection, adherence and invasion
Citation
Bleotu Coralia, Chifiriuc Mariana Carmen, Dracea Olguta, Iordache Carmen, Delcaru Cristina, Lazar Veronica. In vitro modulation of adherence and invasion ability of enteroinvasive escherichia coli by different viruses. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 1359-1363.