Process optimization of L-glutaminase production by Trichoderma koningii under solid state fermentation (SSF).
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Date
2010-11
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Abstract
Solid state fermentation (SSF) was carried out for the production of Lglutaminase
by the fungal strain Trichoderma koningii using sesamum oil cake as the solid
substrate. L-glutaminase has received significant attention in recent years owing to its potential
applications in medicine as an anticancer agent, as an efficient anti-retroviral agent and as a
biosensor. In food industries it is used as a flavor and aroma enhancing agent. The overall
maximum yield of L-glutaminase (19.41 U/gds) was achieved with the optimized process
parameters of initial moisture content 65%, initial pH of the medium 7.0, supplemented with Dmaltose
(1.0% w/v) and malt extract (1.0% w/v), inoculated with 2ml of 6 day old fungal culture
and incubated at 33°C for 5 days. Both physico-chemical and nutritional parameters had played a
significant role in the production of the enzyme, L-glutaminase. The enzyme production was
found to be growth associated with the growth of the fungal culture.
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Keywords
L-glutaminase, Trichoderma koningii, Sesamum oil cake, Solid state fermentation, Process parameters, Optimization
Citation
Pallem Chanakya, Manipati Srikanth, Somalanka Subba Rao. Process optimization of L-glutaminase production by Trichoderma koningii under solid state fermentation (SSF). International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 1168-1174.