Isolation and purification of cuticle degrading extra cellular proteases from entomopathogenic fungal species of beauveria bassiana and metarhizium anisopliae.
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Date
2010-11
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Abstract
Enhanced Pr1 and Pr2 activities were observed in entomopathogenic
fungi Beauveria bassiana (UB9) and Metarhizium anisopliae (UM4) supplemented with
casein. Highest activity was observed at 72 hours of incubation when compared to
minimal medium (MM) and colloidal chitin amended media. The extra cellular proteases
(Pr1 and Pr2) thus isolated were purified through dialysis and their activities were found
to be increased by two-fold. Pr1 and Pr2 were subjected to SDS - PAGE analysis and
activity gel electrophoresis. The molecular weight of protease produced by Beauveria
bassiana (UB9) is 19 KD and that of Metarhizium anisopliae (UM4) is 21 KD.
Description
Keywords
Extracellular proteases, Pr1, Pr2, Beauveria bassiana, Metarhizium anisopliae
Citation
Lakshmi S Bhagya, Kaur S Gurvinder, Palem P C Padmini. Isolation and purification of cuticle degrading extra cellular proteases from entomopathogenic fungal species of beauveria bassiana and metarhizium anisopliae. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 1150-1156.