Heme regulated eukaryotic initiation factor 2α kinase—a molecular indicator of haemolytic anemia.
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Date
1997-06
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Abstract
The heme regulated eukaryotic initiation factor 2α (eIF 2α) kinase, also called the
heme regulated inhibitor (HRI), is a key regulator of protein synthesis in mammalian reticulocyte.
HRI is almost undetectable in blood samples of normal rabbits and it increases by 12 15 fold in the
reticulocytes of anemic rabbits. In order to determine if such an increase in the quantity of HRI is
gradual during anemia, and if it could be an indicator of anemia, we have carried out a detailed
analysis on the expression of HRI and its eIF 2α kinase activity in rabbit reticulocyte lysates during
various stages of acetylphenylhydrazine (APH) induced anemia. In a 9 day schedule of induction of
anemia, using an anti HRI monoclonal antibody, HRI was detectable immediately after completion
of fourth injection (day 5) and it increased gradually during the entire period reaching its maximum
(24 fold) on day 9. Furthermore, when rabbits recovered from anemia due to individual response to
the drug, quantity of HRI decreased significantly. Northern blot analysis results were similar to those
of the Western blot. The other parameters that are generally used to monitor anemia in human
patients, namely, reticulocyte count, haematocrit level and haemoglobin content although changed at
the onset of anemia, did not change significantly during its progression. These results thus indicate
that HRI could be a more appropriate and sensitive indicator of drug induced anemia.
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Keywords
Heme regulated inhibitor, eIF 2α kinase expression, drug induced anemia, molecular indicator
Citation
Anand S, Pal J K. Heme regulated eukaryotic initiation factor 2α kinase—a molecular indicator of haemolytic anemia. Journal of Biosciences. 1997 Jun; 22(3): 287-298.