Immunoprophylactic studies with a 43 kDa human circulating filarial antigen and a cross reactive 120 kDa Brugia malayi sodium dodecyl sulphate soluble antigen in filariasis.
Loading...
Date
1997-01
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Bancroftian filariasis is a major public health problem affecting about 120 million
people all over the world. Immunoprophylaxis may serve as an additional adjunct along with
chemotherapy and anti larval measures for successful filaria control. Circulating filarial antigen
fraction (CFA2-6) containing 43 kDa antigen and adult Brugia malayi sodium dodecyl sulphate
(S DS) soluble antigen fraction BmA-2 with a 120 kDa molecule were earlier shown to be reactive
with endemic normal sera by immunoblotting and indirect ELISA techniques. BmA-2 was
found to be highly cross reactive with CFA2-6. Sera raised against both the antigen fractions
showed about 9 0 % cytotoxicity to the parasites in the presence of jird peritoneal cells in in vitro
as well as by in situ micropore chamber implantation technique. Further in in vivo studies using
animal model, jirds CFA2-6 and BmA-2 could induce about 90% protection to infection in
immunized animals. In passive transfer studies of immunity it has been observed that BmA-2
induced protection is mainly antibody mediated.
Description
Keywords
Filariasis, immunoprophylaxis, cytotoxicity, passive immunization
Citation
Chenthamarakshan V, Cheirmaraj K, Reddy M V R, Harinath B C. Immunoprophylactic studies with a 43 kDa human circulating filarial antigen and a cross reactive 120 kDa Brugia malayi sodium dodecyl sulphate soluble antigen in filariasis. Journal of Biosciences. 1997 Jan; 22(1): 91-98.