Immunoprophylactic studies on cell wall associated proteins of Mycobacterium tuberculosis H37Ra.
Loading...
Date
1997-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The cell wall protein peptidoglycan complex (CW-PPC) of Mycobacterium
tuberculosis H37Ra was isolated through sequential extraction of lipids, carbohydrates
and soluble proteins. CW-PPC emulsified in FIA was found to induce significant protection
in mice against challenge with LD50 dose of M. tuberculosis H37Rv. To identify the
immunoprotective components of CW-PPC, the proteins in avid association with peptidogican
were dissociated by chemical treatment with trifluoromethanesulthonic acid (CF3CO3H):
anisole (2:1). Immunoreactivity of total (CW-Pr) as well as its component proteins i.e.,
71, 60 and 45 kDa proteins of cell wall was studied in animals immunized with CW-Pr-FIA.
The 71 kDa protein was found to be most immunoreactive giving higher T-cell sensitization
and humoral responses. Further, immunization of mice with 71 kDa-FIA demonstrated
enhanced T- and B-cell responses. Mice immunized with 71 kDa-FIA gave significantly
higher protection (P ≤ 0·05) against intravenous challenge with LD50 dose of M. tuberculosis
H37Rv, than BCG immunized animals. The results indicate the potential of 71 kDa cell
wall protein as a suitable candidate for Cthe subunit vaccine.
Description
Keywords
Tuberculosis, cell wall;, protein antigens, vaccine
Citation
Dhiman Neelam, Verma Indu, Khuller G K. Immunoprophylactic studies on cell wall associated proteins of Mycobacterium tuberculosis H37Ra. Journal of Biosciences. 1997 Jan; 22(1): 13-21.