Activities of hydrolytic enzymes in callus cultures of tobacco during organogenesis.
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Date
1995-12
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Abstract
Starch, total sugars, reducing sugars and protein contents and the specific
activities of hydrolytic enzymes such as amylase, Phosphorylase, soluble acid invertase,
wall-bound acid invertase, sucrose synthetase, acid and alkaline phosphatases and
ribonuclease were determined in root forming, shoot forming and non-organ-forming
callus cultures of tobacco. Organ-forming cultures not only showed higher amounts
of the above metabolites but also higher enzyme activities compared to non-organ-forming
cultures. The activities of these enzymes in relation to organogenesis is discussed.
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Keywords
Tobacco callus, hydrolytic enzymes, organogenesis
Citation
Naidu K R, Kishor P B Kavi. Activities of hydrolytic enzymes in callus cultures of tobacco during organogenesis. Journal of Biosciences. 1995 Dec; 20(5): 629-636.