Developmental study of thermotolerance and the heat shock response in Lucilia cuprina (Weidemann).
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Date
1995-06
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Abstract
The ability to withstand thermal stress in a laboratory population of the blowfly
Lucilia cuprina (measured as per cent adult survival following varying periods of exposure
to elevated temperature up to a maximum of 48°C) was in the order pupa > larva > adult.
Pre-exposure to a mild heat shock (37°C) induced tolerance to temperatures which were
otherwise lethal. An analysis of heat shock-induced protein synthesis during development
at similar elevated temperatures presented patterns corresponding to the above observations
on thermotolerance. The induced level of synthesis of major heat shock proteins (viz., 79,
69, 28, 20 and 19 kDa) were greater in larval tissues than in most of the adult tissues
except gonads. The response varied between young (2 days) and old (30 days) adults in
a tissue-specific manner. In general, heat shock protein 69 kDa was most abundant in all
the tissues studied. Control as well as heat shocked Malpighian tubules of adults uniquely
showed two major [35S]methionine labelled bands corresponding to approximately 62 and
64 kDa. Immunoblots showed the 62 kDa protein to cross react with an antibody against
Helioihis HSP60. Although the synthesis of the 62 kDa polypeptide was prominent only
in Malpighian tubules of adult blowflies, nearly equal levels of this HSP60 family polypeptide
were present in all tissues (control as well heat shocked) except the larval salivary glands.
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Keywords
Heat shock response, thermotolerance, Lucilia cuprina, heat shock proteins, tropical adaptation
Citation
Tiwari P K, Mohan D R K, Joshi Archana. Developmental study of thermotolerance and the heat shock response in Lucilia cuprina (Weidemann). Journal of Biosciences. 1995 Jun; 20(3): 341-354.