Role of hydroxyl radical in superoxide induced microsomal lipid peroxidation: Protective effect of anion channel blocker.
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Date
1996-03
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Abstract
Treatment of bovine pulmonary artery smooth muscle microsomes with the superoxide
radical generating system hypoxanthine plus xanthine oxidase stimulated iron release, hydroxyl
radical production and lipid peroxidation. Pretreatment of the microsomes with deferoxamine or
dimethylthiourea markedly inhibited lipid peroxidation, and prevented hydroxyl radical production
without appreciably altering iron release. The superoxide radical generating system did not
alter the ambient superoxide dismutase activity. However, addition of exogenous superoxide
dismutase prevented superoxide radical induced iron release, hydroxyl radical production and
lipid peroxidation. Simultaneous treatment of the microsomes with deferoxamine, dimethylthiourea
or superoxide dismutase prevented hydroxyl radical production and liqid peroxidation.
While deferoxamine or dimethylthiourea did not appreciably alter iron release, superoxide
dismutase prevented iron release. However, addition of deferoxamine, dimethylthiourea or
superoxide dismutase even 2 min after treatment did not significantly inhibit lipid peroxidation,
hydroxyl radical production and iron release. Pretreatment of microsomes with the anion
channel blocker 4,4'-dithiocyano 2,2'-disulphonic acid stilbine did not cause any discernible
change in chemiluminiscence induced by the superoxide radical generating system but markedly
inhibited lipid peroxidation without appreciably altering iron release and hydroxial radical
production.
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Keywords
Anion channels, superoxide radical, hydroxyl radical, iron, microsomes, lipid peroxidation, smooth muscle tissue
Citation
Ghosh Salil K, Chakarborti Tapati, Banerjee Arun B, Roychoudhury Sujata, Chakraborti Sajal. Role of hydroxyl radical in superoxide induced microsomal lipid peroxidation: Protective effect of anion channel blocker. Journal of Biosciences. 1996 Mar; 21(1): 35-43.