Calcium and magnesium induced changes in the relative fluidity of phosphatidylcholine liposomes.
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Date
1990-09
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Abstract
The effect of Ca2+ and Mg2+ on relative fluidity of phosphatidylcholine
liposomes was studied by measuring the degree of chlorophyll fluorescence polarization.
An increase in the degree of fluorescence polarization was observed on incubation of
liposomes with different concentrations of Ca2+ or Mg2+ . The results have been
interpreted on the basis of increase in the size of liposomes which could be brought about
by calcium or magnesium induced fusion of small unilamellar liposomes to form larger
vesicles. Fusion of liposomes has also been confirmed by the experiments on efficiency of
energy transfer from chlorophyll b to chlorophyll a, and transmission electron microscopy
of liposomes before and after incubation with Ca2+ and Mg2.
Description
Keywords
Liposomes, fluidity, fluorescence polarization, phosphatidylcholine
Citation
Mishra R K, Singhal Gauri S. Calcium and magnesium induced changes in the relative fluidity of phosphatidylcholine liposomes. Journal of Biosciences. 1990 Sep; 15(3): 193-197.