In situ study of chorion gene amplification in ovarian follicle cells of Drosophila nasuta.
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Date
1990-06
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Abstract
The temporal and spatial pattern of replication of chorion gene clusters in
follicle cells during oogenesis in Drosophila melanogaster and Drosophila nasuta was
examined by [3H] thymidine autoradiography and by in situ hybridization with chorion
gene probes. When pulse labelled with [3H] thymidine, the follicle cells from stage 10-12
ovarian follicles of both Drosophila melanogaster and, Drosophila nasuta often showed
intense labelling at only one or two sites per nucleus. In situ hybridization of chorion gene
probes derived from Drosophila melanogaster with follicle cell nuclei of Drosophila
melanogaster and Drosophila nasuta revealed these discrete [3H] thymidine labelled sites to
correspond to the two amplifying chorion gene clusters. It appears, therefore, that in spite
of evolutionary divergence, the organization and programme of selective amplification of
chorion genes in ovarian follicle cells have remained generally similar in these two species.
The endoreplicated and amplified copies of each chorion gene cluster remain closely
associated but the two clusters occupy separate sites in follicle cell nucleus.
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Keywords
Chorion genes, amplification, endoreplication, Drosophila, in situ hybridization
Citation
Tiwari P K, Lakhotia S C. In situ study of chorion gene amplification in ovarian follicle cells of Drosophila nasuta. Journal of Biosciences. 1990 Jun; 15(2): 99-105.