5-Methylcytosine content and methylation status in six millet DNAs.
Loading...
Date
1990-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
High performance liquid chromatographic analysis of the total nuclear DNAs
of 6 millets plant species indicates that the 5-methylcytosine content ranges from 3% in
barn yard millet to 9·6% in great millet while the fraction of cytosines methylated varies
between 14% in little millet to 31 % in pearl millet. Digestion of millet DNAs with
MspI/HpaII suggests that CpG methylation is more in great millet DNA while CpC
methylation is more in the other 5 millet DNAs. Digestion of millet DNAs with MboI,
Sau3AI and DpnI indicates that some of the 5’ GATC3’ sequences are methylated at adenine
and/or cytosine residues except in little millet where adenine methylation of the 5’GATC3’
sequences is insignificant and there is a predominance of cytosine methylation in these
sequences.
Description
Keywords
5-Methylcytosine content, cytosine methylation, adenine methylation, millets.
Citation
Kumar Lalitha S, Hendre R R, Ranjekar P K. 5-Methylcytosine content and methylation status in six millet DNAs. Journal of Biosciences. 1990 Mar; 15(1): 47-52.