Analysis, characterization and diagnostic use of circulating filarial antigen in bancroftian filariasis.
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Date
1990-03
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Abstract
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of circulating
filarial antigen fraction-2 isolated from plasma of microfilaraemic patients with Wuchereria
bancrofti infection has shown 21 bands with molecular weights ranging from 12 to
120 kDa. The gel (12 cm) was sliced at an interval of one cm and the eluates of all the
gel slices viz., CFA2–1 to CFA2–12 showed the presence of filarial antigen by sandwich
enzyme-linked immunosorbent assay. The low molecular weight circulating filarial antigen
fractions were found to share a common epitope with Wuchereria bancrofti microfilariae
excretory-secretory antigen and urinary filarial antigen. The 3 antigen fractions CFA2-1,
CFA2–9 and CFA2–12 showed higher sensitivity in detecting filarial immunoglobulin Μ
antibodies than immunoglobulin G antibodies. However CFA2–9 fraction was found
useful in serological differentiation of microfilaraemics from those with disease
manifestations when filarial immunoglobulin G antibodies were detected. The antigenic
epitope of CFA2–1 appears to be a carbohydrate, whereas CFA2–9 appears to be protein
in nature.
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Keywords
Enzyme linked immunosorbent assay, filarial serum immunoglobulin G, circulating filarial antigen, sodium dodecyl sulphate, Polyacrylamide gel electrophoresis
Citation
Parkhe K A, Reddy M V R, Cheirmaraj K, Ramaprasad P, Harinath B C. Analysis, characterization and diagnostic use of circulating filarial antigen in bancroftian filariasis. Journal of Biosciences. 1990 Mar; 15(1): 37-46.