In vivo biocompatibility of aliphatic segmented polyurethane in rabbit.
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Date
1989-09
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Abstract
An aliphatic segmented polyurethane with soft to hard segment ratio 3 was
synthesised using hexamethylene diisocyanate, polypropylene glycol 400 and 1,4-butane
diol.A stainless steel cage implant system has been used to study the in vivo
biocompatibility of this polyurethane. United States Pharmacopoeia negative control
polyethylene was used for the comparison. Three cages, one with polyurethane another
with United States Pharmacopoeia polyethylene and the third control empty cage were
implanted subcutaneously in the dorsal aspect of rabbits. The inflammatory exudate
surrounding the material was aspirated from the cages on 4, 7, 14 and 21 days after
implantation. The total protein content in the exudate aspirated from all the 3 cages was
significantly higher at 7 days than in the reported normal rabbit serum of New Zealand
white rabbit but equal to that of our rabbit colony. The albumin concentration was lower
in the initial period but increased at 21 days post implantation period in all the cages.
Concentration of α1, α2 and γ-globulin also decreased in all cages at 21 days. Neutrophils
were predominant in all the exudates aspirated from polyurethane, polyethylene and
empty control cages during whole implantation period. This is attributed to the profound
effect of the cages on the surrounding vasculature. Macrophage was found to be seen
during acute phase of inflammation due to the migration of macrophage along with
neutrophil towards the inflammatory lesion. The percentage of neutrophils showed a faster
decline in the cage containing polyethylene at 21 days. The extra cellular alkaline
phosphatase activity, though higher in exudate from cages containing polyurethane at 14
days post implantation, was same in all 3 cages at 21 days. Leucine amino peptidase
activity was found to be decreased at 21 days of post implantation time though the empty
control cage exhibited an increase at 14 days post implantation. The inflammatory
response at 21 days was similar in polyurethane and the control polyethylene.
Description
Keywords
Cage implants, aliphatic segmented polyurethane, inflammatory exudate, proteins, leukocytes, enzymes, biocompatibility
Citation
Jayabalan M, Rathinam K, Kumary T V, Mohanty Mira. In vivo biocompatibility of aliphatic segmented polyurethane in rabbit. Journal of Biosciences. 1989 Sep; 14(3): 289-299.