Detection of hepatitis Β virus in blood samples negative for surface antigen, by DNA probe hybridization assay.
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Date
1989-09
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Abstract
A DNA hybridization assay was developed using a cloned hepatitis Β viral
genome to detect the presence of infectious virions in human serum. The merit of this assay
was to put in evidence virus particles in 7 out of 133 sera that were negative for surface
antigen (HBsAg) using routine serological methods. The usefulness of this assay was
confirmed by actual visualization of the virus under electron microscope. Some serum
samples although positive for surface antigen, did not give a hybridization signal by dot
blot assay and might indicate cases of acute hepatitis.
Description
Keywords
DNA dot hybridization, hepatitis Β virus, integration
Citation
Banerjee Kakoli, Sharma Gita, Upadhyay S, Anand B S, Raju G S, Khandekar P S. Detection of hepatitis Β virus in blood samples negative for surface antigen, by DNA probe hybridization assay. Journal of Biosciences. 1989 Sep; 14(3): 279-289.