A rapid procedure for the isolation of lysosomes from kidney cortex by Percoll density gradient centrifugation.
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Date
1989-09
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Abstract
Highly pure lysosomes were isolated from buffalo (Bubalus bubalis) kidney
cortex by a procedure involving differential and isopycnic Percoll density gradient
centrifugations. Arylsulphatase, N-acetyl-ß-glucosamindase and cathepsin D in the
lysosomal preparation were 26-45-fold enriched over the homogenate. The purified
lysosomes contained less than 0·06% of mitochondrial, microsomal and peroxisomal
marker enzymes. In the electron micrographs the particles appeared as large dense
granules of size 0·3–1·9 μm with no apparent structural features belonging to mitochondria
or microsomes. The isolation procedure was also found to be suitable to obtain highly
pure lysosome particles from renal cortex of other sources such as rat, lamb and beef. No
ultracentrifugation steps were involved in the procedure.
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Keywords
Lysosomes; kidney cortex, Percoll gradient, isolation procedure, buffalo kidney
Citation
Harikumar P, Darshini T, Dutt Aditi, Ninjoor V. A rapid procedure for the isolation of lysosomes from kidney cortex by Percoll density gradient centrifugation. Journal of Biosciences. 1989 Sep; 14(3): 269-277.