Relative ability of ovine follicle stimulating hormone and its ß-subunit to generate antibodies having bioneutralization potential in nonhuman primates.
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Date
1989-06
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Abstract
The relative ability of ovine follicle stimulating hormone and its β-subunit, two
potential candidates for male contraceptive vaccine, to generate antibodies in monkeys
capable of bioneutralizing follicle stimulating hormone was assessed using in vitro model
systems. Antiserum against native ovine follicle stimulating hormone was found to be
highly specific to the intact form with no cross-reactivity with either of the two subunits
while the antiserum against β-subunit of follicle stimulating hormone could bind to the β-
subunit in its free form as well as when it is combined with α-subunit to form the intact
hormone. Both antisera could block the binding of the hormone to the receptor if the
hormone was preincubated with the antibody. However, the follicle stimulating hormone
β-antisera could only inhibit the binding of the hormone partially (33% inhibition) if the
antibody and receptor were mixed prior to the addition of the hormone, while antisera to
the native follicle stimulating hormone could block the binding completely (100%
inhibition) in the same experiment. Similarly antisera to the native follicle stimulating
hormone was significantly effective in blocking (100%) response to follicle stimulating
hormone but not the β-subunit antisera (0%) as checked using an in vitro granulosa cell
system. Thus the probability of obtaining antibodies of greater bioneutralization potential
is much higher if intact hormone is used as an antigen rather than its β-subunit as a
vaccine.
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Keywords
Gonadotropins, hormone action, receptor
Citation
Moudgal N R, Sairam M R, Dighe R R. Relative ability of ovine follicle stimulating hormone and its ß-subunit to generate antibodies having bioneutralization potential in nonhuman primates. Journal of Biosciences. 1989 Jun; 14(2): 91-100.