Effects of ovulen-50, diethylnitrosamine and phenobarbital on liver regeneration in female rats.
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Date
1989-03
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Abstract
Short term effects of ovulen-50, a combination type oral contraceptive agent
and phenobarbital—an established hepatic tumour promoter, were examined in the livers
of diethylnitrosamine-initiated and uninitiated female rats. Liver mitotic activity as judged
by liver weight, [3H] thymidine incorporation into DNA and levels of DNA, RNA and
protein were measured in non-regenerating and regenerating liver. Hepatic γ-glutamyl
transpeptidase activity and hepatocyte agglutination with concanavalin A were examined
in diethylnitrosamine- and/or phenobarbital-treated rats.
The results indicate that diethylnitrosamine or ovulen-50 individually are mitoinhibitory
in regenerating liver. Phenobarbital alone has a slight mitostimulatory effects in nonregenerating
liver, but no effect on liver regeneration. Administration of ovulen-50 and
phenobarbital to diethylnitrosamine initiated rats mitigated the mitoinhibition during
regeneration. Contrary to the earlier observation with ovulen-50, neither phenobarbital
nor diethylnitrosamine induced hepatocyte agglutination in the presence of concanavalin A.
Like ovulen-50, diethylnitrosamine also increased the level of hepatic γ-glutamyl
transpeptidase. Phenobarbital produced only insignificant rise and did not substantially
exacerbate the effect diethylnitrosamine.
The data show that though some of the effects of ovulen-50 resemble those of
diethylnitrosamine or phenobarbital, the changes observed may not be related to the
neoplastic phenomenon since they were not seen in an initiator-promoter combination
regimen.
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Keywords
Oral contraceptive agent, phenobarbital, diethylnitrosamine, liver regeneration, nucleic acids, γ-glutamyl transpeptidase, concanavalin A, hepatocytes agglutination
Citation
Annapurna V V, Mukundan M A, Sesikeran B, Bamji Mahtab S. Effects of ovulen-50, diethylnitrosamine and phenobarbital on liver regeneration in female rats. Journal of Biosciences. 1989 Mar; 14(1): 1-7.