Analysis of nuclear proteins from silk glands of Bombyx mori.
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Date
1988-12
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Abstract
A gentle method for the isolation of nuclei from developing silk glands of
Bombyx mori has been standardized. The nuclei, whether isolated or directly visualized
in situ within the silk glands, exhibit complex morphology. The nuclei occupy almost the
entire volume of the gigantic silk gland cells. Although the isolated nuclei still retain their
ramified morphology, being polyploid they are fragile and often become fragmented. The
histone and low-salt-extractable proteins from nuclei isolated from the middle and
posterior silk glands on different days of the fourth and fifth instars of larval development
have been analysed. The histones did not show any stage- or tissue-specific variations
whereas the low-salt-extractable proteins showed some developmental stage specific
variation. Using the antibody raised against one such protein, its absence in the early stage
of development has been confirmed by Western blotting techniques. This developmental
stage specific protein may be functionally linked to some activities responsible for boosting
up the production of silk or silk-related proteins during the fifth instar of larval development.
Description
Keywords
Nuclei isolation, ramified nuclei, developmental stage specific proteins, histones, silk gland nuclei, silk gland proteins
Citation
Agarwal Pushpa, Gopinathan K P. Analysis of nuclear proteins from silk glands of Bombyx mori. Journal of Biosciences. 1988 Dec; 13(4): 379-391.