Expression from symbiotic promoters of Rhizobium meliloti in Azotobacter vinelandii and Azospirillum brasilense.
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Date
1988-09
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Abstract
In Rhizobium meliloti, the promoter P1 of the nif HDK operon, and also the
promoter P2, have earlier been shown to be active in the bacteria present in alfalfa root
nodules, but not in the bacteria grown aerobically in culture. Here we have looked at the
expression from P1 and P2 in two non-symbiotic nitrogen-fixing bacteria, Azotobacter
vinelandii and Azospirillum brasilense, using constructions in which the promoters are fused
upstream of the ß-galactosidase gene. The promoter P1, but not P2, is active in A. vinelandii,
while neither P1 nor P2 is active in Azospirillum brasilense.
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Keywords
Rhizobium meliloti, Azotobacter vinelandii, Azospirillum brasilense, P1 and P2, promoters
Citation
Hoare Sarasija, Das H K. Expression from symbiotic promoters of Rhizobium meliloti in Azotobacter vinelandii and Azospirillum brasilense. Journal of Biosciences. 1988 Sep; 13(3): 317-321.