Use of epoxysepharose for protein immobilisation.
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Date
1986-09
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Abstract
Epoxy Sepharose, an activated affinity matrix which has been used for
immobilisation of carbohydrates has been tried for immobilisation of proteins. Under
normal conditions of coupling at neutral or alkaline pH proteins do not couple to epoxy
Sepharose. However, a very high salt concentration during coupling allows the binding of
proteins to epoxy Sepharose at a pH as low as 8·5. Increasing ionic strength and/or pH
facilitates the binding. The bioactivity of the proteins is not destroyed by the
immobilisation. This matrix, unlike cyanogen bromide-Sepharose, retains its ability to
bind albumin by 80–90% even after 60 days of storage in aqueous suspension at 4°C. Its
capacity to bind proteins is comparable to that of cyanogen bromide-Sepharose.
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Keywords
Epoxy Sepharose, affinity matrix, protein immobilisation
Citation
Murthy G S, Moudgal N R. Use of epoxysepharose for protein immobilisation. Journal of Biosciences. 1986 Sept; 10(3): 351-358.