Effect of supplementation with exogenous fatty acid on the biological properties of a fatty acid requiring auxotroph of Salmonella typhimurium.
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Date
1986-09
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Abstract
The effects of changes in fatty acid composition of the cell membrane on
different biological functions of Salmonella typhimurium have been studied with the help
of a temperature sensitive fatty acid auxotroph which cannot synthesise unsaturated fatty
acids at high temperature. On being shifted to nonpermissive temperature the cells
continue growing for another one and half to two generations. The rates of protein and
DNA syntheses run parallel to the growth rate but the rate of RNA synthesis is reduced.
Further, there is a gradual reduction in the rate of transport of exogenous uridine and
thymidine into the soluble pool. The transport process can be restored by supplementing
the growth medium with cis-unsaturated fatty acids but not trans-unsaturated ones
although the growth of the cells is resumed by supplementation with either cis or
trans-unsaturated fatty acids. However, supplementation with trans, trans-unsaturated
fatty acids leads to only partial recovery of the transport process. The rate of oxygen
uptake is also affected in cells grown in the presence of the trans-unsaturated fatty acids,
elaidic acid and palmitelaidic acid. Analysis of cells grown under different fatty acid
supplementation indicate that fatty acid composition of the cell membrane, especially the
ratio of unsaturated to saturated fatty acids varies with temperature shift and
supplementation of the growth media with fatty acids.
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Keywords
Transport, fatty acid auxotroph, cis- and trans-unsaturated fatty acids
Citation
Deb J K, Biswas S K, Chakrabarti P, Chakravorty M. Effect of supplementation with exogenous fatty acid on the biological properties of a fatty acid requiring auxotroph of Salmonella typhimurium. Journal of Biosciences. 1986 Sept; 10(3): 335-349.