Preformed messengers in Microsporum canis macroconidia.
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Date
1986-03
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Abstract
Macroconidia of Microsporum canis, when placed in a nutrient medium produce
germ tubes within 4–6 h. Precursor incorporation studies showed that protein synthesis
occurred prior to RNA synthesis. Sucrose density gradient analysis of wet and dry spore
extracts revealed the presence of 16 % and 11 % polysomes respectively. The polysomal content
increased to about 50% within 15 min of germination. Synthesis of RNA occurred only after
2 h of germination. Pool equilibration of the radioactive precursors was not limiting to these
measurements. Polyadenylated RNA was isolated from macroconidia and was found to
comprise 2-2·5 % of the total RNA. The poly(A)+ RNAs were heterodisperse and translatable
in a wheat germ cell free translating system. It was concluded that macroconidia of
Microsporum canis contain pre-formed mRNA which is translated early in germination.
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Keywords
Microsporum canis, preformed mRNA, poly(A)+ RNA, germination, polysomes, RNA/protein synthesis
Citation
Banumathi N, Jayaram B M, Rao G Ramananda. Preformed messengers in Microsporum canis macroconidia. Journal of Biosciences. 1986 Mar; 10(1): 117-126.