Effect of steroid hormones on vaginal epithelial cells: An in vitro model for steroid hormone action.
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Date
1987-09
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Abstract
Conditions have been standardized to maintain rat vaginal epithelial cells in vitro
with more than 95% viability. Cultured epithelial cells were used to study the effects of normal
fetal calf scrum, estradiol and progesterone on the incorporation of [3H]-uridine in RNA
and incorporation of [l4C]-aminoacids in proteins. While fetal calf serum and estradiol
stimulate the incorporation of both uridine and afno acids, progesterone did not show any
effect. Estradiol treated vaginal cells show typical fcroridges (indicative of keratinization
of cells) in contrast to estradiol deprived cells, which show microvilli on cell surface when
examined in scanning electron microscope.
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Keywords
Steroid hormones, vaginal epithelial cells, fetal calf serum
Citation
S Vijayasaradhi, Khar A, Gupta P D. Effect of steroid hormones on vaginal epithelial cells: An in vitro model for steroid hormone action. Journal of Biosciences. 1987 Sept; 12(3): 257-265.