Cloning and characterization of a DNA repair gene in Haemophilus influenzae.
Loading...
Date
1987-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Using a high-efficiency DNA cloning vector pJ1-8, a DNA repair gene uvr1 has
been self-cloned in bacterium Haemophilus influenzae. Chimeric plasmid pKuvrl, carrying
wild type allele of uvr1 gene and flanking DNA sequences, specifically complements a uvrl
gene mutation in the bacterial chromosome. A uvr1– mutation could be transferred from
chromosome by in vivo recombination to pKuvr1 and isolated and designated as plasmid
pKuvrl –. Plasmid pKuvrl carries a 11·3 kb chromosomal DNA insert which was scanned
for the presence of any other DNA repair genes by a novel method of directed mutagenesis.
Preliminary analysis of the 3 new mutants isolated by this method supports the notion that
the insert contains more than one gene concerned with ultraviolet radiation-sensitivity.
Description
Keywords
Haemophilus influenzae, self-cloning, uvr1 gene, directed mutagenesis
Citation
Kanade Rohinee P, Notani N K. Cloning and characterization of a DNA repair gene in Haemophilus influenzae. Journal of Biosciences. 1987 Jun; 12(2): 115-123.