Effects of protein deficiency on selective elicitation of lysosomal enzymes in rat peritoneal macrophages.
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Date
1985-09
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Abstract
Young albino rats were fed ad libitum 4, 8 or 20 % (control) protein diet for 1–4
weeks. Total activities of some of the lysosomal enzymes, namely, acid phosphatase, aryl
sulphatase, ß-glucuronidase and cathepsin D, were determined in resident and proteasepeptone
elicited peritoneal macrophages. Total cell number, protein content and the lysosomal
enzyme activities were increased significantly in protease-peptone elicited macrophages;
though at a lower rate in 4 % protein-fed group compared to control ones. However, the rate of
induction of the tested hydrolases was selective and their response to the stimulant varied widely.
Similarly, response of each enzyme to low protein diet also varied. Thus, at 4 weeks, cathepsin
D and ß-glucuronidase activities, expressed per total number of elicited macrophages were
reduced by 45 and 60 %, respectively, in 4 % protein-fed animals. These results indicate that the
metabolic events related to lysosomal function in macrophages, are affected by dietary
restriction of proteins.
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Keywords
Rat, protein deficiency, peritoneal macrophages, elicitation, lysosomal enzymes
Citation
Iyengar U R, Vakil U K. Iyengar U R, Vakil U K. Effects of protein deficiency on selective elicitation of lysosomal enzymes in rat peritoneal macrophages. Journal of Biosciences. 1985 Sept; 9(1&2): 23-33.