Genetic transformation in bacteria.
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Date
1984-10
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Abstract
Certain species of bacteria can become competent to take up high molecular weight
DNA from the surrounding medium. DNA homologous to resident chromosomal DNA is
transported, processed and recombined with the resident DNA. There are some variations in
steps leading to transformation between Gram-positive bacteria like biplococcus pneumoniae
and Gram-negative bacteria represented by Haemophilus influenzae but the integration is by
single-strand displacement in both cases. Plasmid (RSF0885) transformation is low in
Haemophilus influenzae but this is increased significantly if (homologous) chromosomal DNA
is spliced to plasmid DNA. In Haemophilus influenzae, rec1 function is required for peak
transformation with chimeric plasmids. Chimeric plasmid fixed presumably extrachromosomally
undergoes frequent recombination between homologous segments contained in resident
chromosome and the plasmid.
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Keywords
DNA-cell interaction, competence, specificity in DNA uptake, single-strand displacement, chimeric plasmid transformation, rec1 function, recombination
Citation
Notani N K, Joshi V P, Kanade R P. Genetic transformation in bacteria. Journal of Biosciences. 1984 Oct; 6(4): 525-533.