Isolation and characterization of plasma membrane from Acanthamoeba culbertsoni.
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Date
1985-06
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Abstract
A rapid method for preparation of plasma membrane from Acanthamoeba
culbertsoni involving toluene treatment followed by lithium bromide extraction is described. In
the plasma membrane preparation, 5'-nucleotidase, Na+ + K+ -ATPase, Mg2+ -ATPase and
glucose-6-phosphatase activities were enriched. The membrane preparation was free from
nucleic acid, cytochrome P-450 and cytochrome b5. Amino acid (14C-Ieucine) was not
incorporated in the plasma membrane in 2 min. Succinic dehydrogenase was not detectable in
the plasma membrane preparation. The molar ratio of cholesterol and phospholipids was 0ยท95
which is characteristics for plasma membranes. Under electronmicroscopy the preparation
was homogenous without any other component of the cell. Plasma membrane proteins and
glycoproteins were separated on acrylamide gel electrophoresis.
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Keywords
Amoeba, plasma membrane, enzymes
Citation
Mishra Sanjay Kumar, Garg N K, Kidwai A M. Isolation and characterization of plasma membrane from Acanthamoeba culbertsoni. Journal of Biosciences. 1985 Jun; 7(3&4): 365-369.